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[Optimization of the conditions for electroporation and the addition nisin for Pseudomonas aeruginosa inhibition]

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A mathematical approach was applied in order to optimize the effect of electroporation by application of pulsed electric fields (PEF) and nisin addition on the inhibition of a strain of Pseudomonas aeruginosa isolated from river sediments. This strain showed to be highly resistant to nisin as only two log cycles reduction of viable cells were obtained in the presence of 84,000 IU/ml nisin. But when a combination of bacteriocin and selected PEF treatment conditions were applied, 4.4-decimal log cycle reduction could be achieved. PEF and nisin interaction seems to be complex, as at lower electric field intensities (i.e., 5 kV/cm) an increment in the number of pulses applied clearly induced a lower inhibitory effect of nisin. At higher PEF intensities (i.e., 11 kV/cm), the inhibitory effect of nisin increased with the number of pulses applied. Results overall, the obtained indicate the possibility of combining PEF and nisin treatments in order to improve the inhibition of resistant microorganisms. The Doehlert experimental design and surface response methodology was an interesting tool to obtain or predict the optimal combination of the stress factors applied.

Author:

Santi, L. and Cerrutti, P. and Pilosof, A. M. and de Huergo, M. S.

Year:

2003

Volume:

35

Pages:

198-204--

Keywords:

Dose-Response Relationship, Drug Electromagnetic Fields Electrophoresis, Gel, Pulsed-Field Electroporation/*methods Microbial Sensitivity Tests/*methods Models, Theoretical Nisin/administration & dosage/*pharmacology Pseudomonas aeruginosa/*drug effects/isolation & purification Regression Analysis Water Microbiology

Comment:

Santi, L Cerrutti, P Pilosof, A M de Huergo, M S Comparative Study English Abstract Argentina Revista Argentina de microbiologia Rev Argent Microbiol. 2003 Oct-Dec;35(4):198-204.

Address:

Facultad de Ingenieria, Universidad de Buenos Aires, Pabellon de Industrias, Ciudad Universitaria, 1428 Buenos Aires, Argentina.

Book title:

Rev Argent Microbiol

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